The film was a biopic called "Midnight Rider," and it was supposed to star William Hurt as former Manatee County resident Gregg Allman.
Filming had just begun when the crew members were struck Feb. 20.
Investigators say the crew was on the tracks without the railroad's permission. The crew was filming on a bridge and second camera assistant Sarah Jones could not escape when when the train barreled into the set.
There's no word yet on whether the filmmakers will resume the project later.
Tyson Ritter of the All-American Rejects was set to play young Gregg Allman in the film. The cast also included Eliza Dushku, Bradley Whitford and Wyatt Russell, the son of Kurt Russell and Goldie Hawn, as Duane Allman.